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Reputable Mentor II
Reputable Mentor II
Ioanna Ntai1, Anson Pierce2, Paul Gulde2, Martin Samonig3, John Brann4, Christopher Elicone4, Amanda Souza1, Ralf Tautenhahn1, Daniel Lopez Ferrer1, and Andreas Huhmer1
ASMS 2019 Poster
Purpose: Measure nutrient limitations or metabolite buildup during cell culture to determine optimized cell media for improved antibody production. Methods: Separate cultures of CHO cells expressing the same recombinant antibody were supplemented with 6 different feeds, in 3 bioreactors per feed. A Thermo Scientific™ Transcend™ Duo LX-2 UHPLC system was coupled to a Thermo Scientific™ Orbitrap ID-X™ Tribrid™ mass spectrometer and extracellular metabolites were analyzed in both positive and negative mode. Data were processed using Thermo Scientific™ Compound Discoverer™ software suite for unknown identification and differential analysis. Results: This dual LC/MS system was successfully applied in the differential analysis of cell media with increased throughput, without compromising reproducibility or compound detection.

1Thermo Fisher Scientific, San Jose, CA 2Thermo Fisher Scientific, Grand Island, NY 3Thermo Fisher Scientific, Germering, Germany 4Thermo Fisher Scientific, Franklin, MA
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‎10-15-2021 11:31 AM
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