Showing results for 
Search instead for 
Did you mean: 

High-sensitivity TFA-free LC-MS for profiling histones

Reputable Mentor II
Reputable Mentor II
You J, Wang L, Saji M, Olesik SV, Ringel MD, Lucas DM, Byrd JC, Freitas MA.
Proteomics. 2011 Aug;11(16):3326-34.
The analysis of proteins by RPLC commonly involves the use of TFA as an ion-pairing agent, even though it forms adducts and suppresses sensitivity. The presence of adducts can complicate protein molecular weight assignment especially when protein isoforms coelute as in the case of histones. To mitigate the complicating effects of TFA adducts in protein LC-MS, we have optimized TFA-free methods for protein separation. Protein standards and histones were used to evaluate TFA-free separations using capillary (0.3 mm id) and nanoscale (0.1 mm id) C(8) columns with the ion-pairing agents, formic acid or acetic acid. The optimized method was then used to examine the applicability of the approach for histone characterization in human cancer cell lines and primary tumor cells from chronic lymphocytic leukemia patients.
Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA.
Version history
Last update:
‎10-15-2021 11:28 AM
Updated by: