Khatereh Motamedchaboki1, Maowei Dou1, Yongzheng Cong2, Romain Huguet1, Aaron M Robitaille1, Yufeng Shen3, Geremy Clair,4 Charles Ansong,4 Daniel Lopez-Ferrer1, Ryan T. Kelly2,4, and Ying Zhu4
Hupo 2019
Understanding heterogeneity at single cell level is of great interest for biomedical research. MS-based proteomics is a promising technique for single cell analysis by enabling identification and quantification of thousands of proteins in unbiased manner. However, due to inefficient single cell isolation, large sample losses during sample preparation and low throughput, the extension to single cell studies has been largely ineffective. To address these challenges, we combined nanoPOTS (Nanodroplet Processing in One-pot for Trace Samples) technology with Thermo Scientific™ Tandem Mass TagTM (TMTTM) isobaric labeling to efficiently process and analyze single mammalian cells containing <0.2 ng total proteins on new Thermo Scientific™ Orbitrap Eclipse™ Tribrid™ mass spectrometer with Real-Time Search to improve single cell proteome coverage and enhance quantification accuracy.
1 Thermo Fisher Scientific, San Jose, CA
2 Brigham Young University, Provo, UT
3 CoAnn Technologies LLC, Richland, WA
4 Pacific Northwest National Laboratory, Richland, WA, USA
United States
