Murphy K, Bromirski M, Espourteille FA.
ASMS 2012 Poster
Purpose: To implement high resolution accurate mass detection for the simultaneous analysis of multiple components in a high throughput screening assay.
Methods: Several peptides commonly monitored for cancer research were analyzed at various concentration levels in HEPES buffer solutions to determine signal response, linearity,-reproducibility, and Z’ value.
Results: Full scan mode at a resolving setting of 70,000 was utilized for the analysis of several thousand sample sets at cycle time of 30 seconds per sample. Four individual peptides were simultaneously analyzed with results demonstrating a linear signal response across the working range of the assay with analyte sensitivity in the low nM range. Full Scan operation at 70,000 resolving power enabled for data collection across a wide mass range allowing for multiple peptide monitoring at multiple charge states and detection of unexpected sample changes with no impact on instrument performance.
Thermo Fisher Scientific