Murphy K, Duczak N, Espourteille F, Kapron J.
Application Note 532
High throughput screening (HTS) is a valuable part of identifying new leads and directing drug design in pharmaceutical research. The ability to quickly perform large numbers of analyses is critical to the success of any HTS assay. Traditionally, the quantitative components of these analyses are performed using fluorescence or radiolabeled techniques. While these techniques provide for rapid sample analysis, they also contain inherent limitations including the occurrence of false positives, increased time for method development, and costly and complex sample preparation. High-resolution, accurate mass (HRAM) liquid chromatography-mass spectrometry (LC/MS) coupled with chromatographic multiplexing technology provides a selective and sensitive alternative to traditional methods with comparable analytical speed and without the need for labeled substrates. Chromatographic multiplexing coupled with HRAM analysis was used to collect full scan MS data providing valuable spectral information in a high throughput format that is not possible when using traditional targeted MRM experiments.
Thermo Fisher Scientific