Reversed phase (RP) peptide separations are widely used in the biopharmaceutical industry. It is at present one of the most common analytical tools for the characterization of protein therapeutics, which are enzymatically digested. Peptide mapping provides confirmation of the correct amino acids sequence, and play a crucial role in the control of critical quality attributes. Furthermore, RP peptide separations are used in stability study or other fingerprinting based analysis, where the chromatogram of a tested protein digest is compared to a reference sample.
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