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High Resolution Orbitrap Mass Spectrometry for the Analysis of Deuterium-Labeled Lipids in E. Coli

Reputable Mentor II
Reputable Mentor II
Anastasia Kalli1, Cajetan Neubauer2, Tonya Pekar Second1, David Peake1 and Andreas Huhmer1
ASMS 2017 Poster
Employ ultra-high resolution at the MS/MS level for resolving deuterium from 13C peak in deuterium labeling experiments Utilize Thermo Scientific™ LipidSearch™ for lipid identification in E.coli lipid extracts Chromatographic separation was performed with reverse-phase chromatography on a C30 column Mass spectrometric analysis was performed on a Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer operated at a resolution of 120,000 to 500,000 (FWHM at m/z 200) for the MS/MS scans Ultra-high resolution at the MS/MS scans allowed baseline separation of deuterium and 13C isotopes in deuterium labeled lipids extracted from E.coli

1 Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA 95134, USA 2 California Institute of Technology, 1200 E. California Blvd. Pasadena, CA 91125
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Last update:
‎10-15-2021 07:41 AM
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