Zhang Y, Hao Z, Kellmann M, Huhmer A.
ASMS 2012 Poster
Purpose: The performance of a novel hybrid quadrupole-OrbitrapTM mass spectrometer was evaluated in targeted peptide quantification.
Methods: Two high-resolution, accurate-mass (HR/AM) targeted quantification methods were used to analyze 14 peptide standards spiked into a complex E. coli digest background. The data were automatically processed using specialized software.
Results: A detection limit of 10 amol and a linear dynamic range of 4 orders were obtained with msx tSIM methods in a moderately complex background. A detection limit of 10–50 amol and a linear dynamic range of 3–4 orders were obtained with tHCD methods in a highly complex background.
Thermo Fisher Scientific
United States
