on 06-19-201702:46 AM - edited on 10-15-202112:00 PM by Closed Account
Christa Feasley1, Fred Zinnel2, Julian Saba3, Stuart McCorrister4, Garrett Westmacott4 and Grant McClarty4, Chris Grant4 ASMS 2017 Poster Purpose: Examination of the role the chlamydial protease-like activity factor targets has in altering protein expression and relative abundance of post translational modification by monitoring global glycopeptide changes.
Methods: Hela 229 cells were infected with Chlamydia trachomatis L2 RST5 CPAF-sufficient strain and RST17 CPAF-deficient strain, enriched for glycopeptides and analyzed on a Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer. Thermo Scientific™ Proteome Discoverer™ software with Byonic node was used for data analysis.
Results: Overall we identified over 5000 unique intact glycopeptides translating into over 600 unique glycoproteins. Number of unique glycopeptides/glycoforms with levels greater than two-fold change were observed upon infection with Chlamydia trachomatis L2 RST5 CPAF-sufficient and -deficient strain
1 Thermo Fisher Scientific, West Palm Beach, FL, USA
2 Thermo Fisher Scientific, Somerset, NJ, USA
3 Thermo Fisher Scientific, Mississauga, ON, Canada; 4National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada