on 07-10-201309:11 AM - edited on 11-09-202101:39 AM by usermigration2
Saba J, Southwick K, Eliuk S, Zabrouskov V, Snovida S. ASMS 2013 Poster Note Purpose: To demonstrate the use of HCD MS3 for glycan structural elucidation on a novel hybrid mass spectrometer, based on mass resolving quadrupole, Thermo Scientific™ Orbitrap™ analyzer, collision cell, linear ion trap architecture.
Methods: HCD MS2 and MS3 spectra of permethylated chicken ovalbumin glycans were acquired on a Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer. Structural elucidation was performed using SimGlycan® software
Results: The combination of permethylation, HCD MS3 and SimGlycan software enabled successful identification and differentiation of structural isomers of chicken ovalbumin-released glycans