Glycan Structural Elucidation On A Novel Quadrupole Dual Cell Linear Ion Trap Orbitrap Hybrid Mass Spectrometer

[Orbitrap_SciLib]

Saba J, Southwick K, Eliuk S, Zabrouskov V, Snovida S.
ASMS 2013 Poster Note
Purpose: To demonstrate the use of HCD MS3 for glycan structural elucidation on a novel hybrid mass spectrometer, based on mass resolving quadrupole, Thermo Scientific™ Orbitrap™ analyzer, collision cell, linear ion trap architecture. Methods: HCD MS2 and MS3 spectra of permethylated chicken ovalbumin glycans were acquired on a Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer. Structural elucidation was performed using SimGlycan® software Results: The combination of permethylation, HCD MS3 and SimGlycan software enabled successful identification and differentiation of structural isomers of chicken ovalbumin-released glycans


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