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Global in-depth quantitative proteomic analysis of HIV infected cells using a novel Q-OT-qIT mass spectrometer

Reputable Mentor II
Reputable Mentor II
Eliuk S, Johnson J, Zabrouskov V, Krogan N
ASMS 2013 Poster
Purpose: To determine the quantitative changes in the phosphoproteome of human cell lines following infection by the human immunodeficiency virus (HIV) to characterize the HIV-human interface. Methods: Global identification and quantification of changes in human phosphoproteome in response to HIV was performed using stable isotope labeled (SILAC) Jurkat cells and HILIC/TiO2 fractionation and enrichments using the new Orbitrap FusionTM TribridTM mass spectrometer and EASY-ETDTM source. Results: More phosphopeptides were identified using a combination of ETD and CID fragmentation in a single run (via novel data dependent decision tree with dynamic scan management) than when using CID fragmentation alone which permitted precise localization of phosphorylation more frequently.

Thermo Fisher Scientific
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Last update:
‎10-15-2021 10:43 AM
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