Each time mobile or stationary phases differ from one method to another, they have to be exchanged. Furthermore, flushing the system and equilibrating it to the new conditions of the next method is required. Most often, these steps are done manually. Thermo Scientific™ Dionex™ UltiMate™ 3000 Dual LC systems with Automated Application Switching configuration allow users to easily automate switching from one analytical method to the other within only a few minutes when combined with Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System (CDS) software. Hence, Automated Application Switching can be performed overnight or even during the weekends without any manual interaction, allowing for maximum instrument utilization.