on 12-15-202001:07 AM - edited on 10-15-202109:52 AM by Closed Account
Yiran Liang, Hayden Acor, Michaela A. McCown, Andikan J. Nwosu, Hannah Boekweg, Nathaniel B. Axtell, Thy Truong, Yongzheng Cong, Samuel H. Payne, Ryan T. Kelly International Hupo 2020 Deep proteome profiling of sub microgram and nanogram samples is still a technical challenge. The nanoPOTS platform1 has greatly improved the sensitivity of nanogram and single-cell proteomics, but dissemination to other laboratories has been hindered by its cost and complexity. Here we report a full automated processing and analysis platform for low-nanogram proteomics and extending to single cells that usesonly commercially available parts. Termed autoPOTS, this platform should be readily implemented in any proteomics laboratory.