on 06-05-201611:53 AM - edited on 10-15-202111:44 AM by AnalyteGuru
Scheffler K, Damoc E, Sutton J, Bailey A, Houel S, Josephs J. ASMS 2016 Poster Here we cover the three major workflows for characterization of biopharmaceuticals on one single instrument LC-MS platform using single and mixed antibody samples: 1)intact mass analysis, 2) subunit analysis and 3) peptide mapping - aiming at confirming antibody sequences and disulfide bonds, elucidating modifications and probing for scrambled disulfide bonds.
The new High Mass Range mode now also allows for analysis of antibodies and antibody drug conjugates under native conditions requiring a higher mass range up to m/z 8000.
Excellent mass accuracy, resolution and sequence coverages are obtained for results at all stages of the workflows: intact molecular masses, masses of subunits, top-down fragments of subunits as well as peptides providing high confidence results.
For Infliximab unusually low Lys-truncation was observed and confirmed at all molecular levels: in the fully intact mAb, in the Fc subunit and at the peptide level.