Field-free transmission geometry atmospheric pressure matrix-assisted laser desorption/ionisation for rapid analysis of unadulterated tissue samples

[Orbitrap_SciLib]

Trimpin S, Herath TN, Inutan ED, Cernat SA, Miller JB, Mackie K, Walker JM.
Rapid Commun Mass Spectrom. 2009 Sep;23(18):3023-7.
Tissue imaging has the potential to be immensely useful in areas as diverse as detecting cancer boundaries and mapping signaling molecules in brain tissue. In order to retain the information on the location and assembly of the molecular makeup of the tissue, the materials should stay unadulterated to the largest extent possible. The sample preparation and acquisition conditions, especially the use of solvent and vacuum, may become detrimental. Here, we report the analysis of mouse brain tissue by solvent-free matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) using a field-free (FF) transmission geometry (TG) atmospheric pressure (AP) source design.

http://onlinelibrary.wiley.com/doi/10.1002/rcm.4213/abstract
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA