A fast HPLC method and an efficient xtraction and analysis method for montelukast in human plasma has been developed using a Thermo Scientific™ SOLA™ SAX SPE 96 well plate and a Thermo Scientific™ Accucore™ C8 HPLC column. The sample preparation was fast, reproducible, and accurate. The chromatographic method provides analysis time of less than 1.5 minutes.