To describe a high-resolution, high-speed separation of monoclonal
antibody (mAb) fragments using a supermacroporous, reversed-phase
column. Baseline separations of light chain (LC) and heavy chain (HC),
crystallizable fragment (Fc) and antigen-binding fragment (Fab), single chain Fc (scFc) and F(ab')
2 were achieved for rituximab, trastuzumab, infliximab,
and bevacizumab using a 10 minute gradient with water/acetonitrile/formic
acid/trifluoroacetic acid mobile phases.
United States
