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Extending Capabilities of Real-Time Database Search on the Orbitrap Eclipse Tribrid Mass Spectrometer for Multiplexed Proteomics

Reputable Mentor II
Reputable Mentor II
Yang Liu, William D. Barshop, Amirmansoor Hakimi, Jesse D. Canterbury, Romain Huguet, Rosa Viner
International Hupo 2020
To evaluate recent improvements to Real-Time Search for TMT SPS MS3 quantitation workflow including: 1) Close-out to prevent acquisition of excess MS3 for proteins which have already reached a sufficient depth of quantitative sampling, 2) Online false discovery rate (FDR) assessments to predicate MS3 events on real-time PSM-level FDR estimates.

Thermo Fisher Scientific, San Jose, CA, USA
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Last update:
‎10-15-2021 10:52 AM
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