Rosati S, Rose RJ, Thompson NJ, van Duijn E, Damoc E, Denisov E, Makarov A, Heck AJ.
Angew Chem Int Ed Engl. 2012 Nov 22.
Antibody profiling: Native mass spectrometry analysis of intact antibodies can be achieved with improved speed, sensitivity, and mass resolution by using a modified orbitrap instrument. Complex mixtures of monoclonal antibodies can be resolved and their glycan "fingerprints" can be profiled. Noncovalent interactions are maintained, thus allowing antibody-antigen binding to be measured.
http://onlinelibrary.wiley.com/doi/10.1002/anie.201206745/abstract;jsessionid=9F9A3FA452BE9F69F54343...Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH (The Netherlands) bioms.chem.uu.nl.
United States
