Evaluation of Drug Microsomal Stability Using Ultra-High Throughput Laser Diode Thermal Desorption Combined With a High Resolution Mass Spectrometer

[Orbitrap_SciLib]

Duczak N, Picard P, Cook K.
ASMS 2012 Poster
Purpose: To evaluate a high throughput sample introduction technology on a benchtop high resolution mass spectrometer for eADME applications. Methods: For LDTD analysis, sample dilution (1:20 in 75% methanol/water) was performed and 3 μL of solution was deposited onto a Laz-well™ sample plate for analysis via laser desorption on a benchtop quadrupole Orbitrap mass spectrometer. The percent remaining of each sample at each time point was calculated and the halflife of each compound was estimated using Thermo Scientific QuickCalc data review software. Results: Analysis time per sample improved by 7.5 fold when compared to a conventional LC method (20 seconds per sample compared to 3 minutes). Utilizing a generic HR/AM detection method removed the need for MS method development.


Thermo Fisher Scientific