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Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD

Team TFS
Team TFS
In this study, the glycosylation of human transferrin (as a model of PSA) and PSA are investigated by HPAE-PAD using <10 μg of protein. Two methods are utilized to evaluate protein glycosylation. One method is used to investigate the N-linked oligosaccharides and glycan sialylation. A second method evaluates the potential presence of O-linked glycans by monitoring monosaccharide composition. By using a combination of acid hydrolysis and enzymatic digestion steps prior to HPAE-PAD analysis, information about the glycosylation, as well as linkages within the glycans, is determined.
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Last update:
‎07-23-2015 07:59 AM
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