on 06-21-201708:56 AM - edited on 10-15-202107:20 AM by AnalyteGuru
Hai Tuan Pham1, Doreen Kirchberg1, Martin Buratti1, Simon Schafferer1, Barbara Wolf1, Glenn Damkroeger2, Madalina Oppermann2, Anastasia Kalli2, Reiko Kiyonami2, David Peake2, Andreas Huhmer2, Therese Koal1 ASMS 2017 Poster Multiplexed analysis by targeted metabolomics provides a quantitative snapshot of the metabolic phenotype. Targeted and standardized assays are used in research to improve biological insights, monitor disease biomarker signatures, and enable efforts in personalized medicine. A standardized, quantitative, rapid targeted metabolomics approach (AbsoluteIDQ® p400 HR Kit) covering the metabolites necessary for the assessment of the central metabolic pathways based on amino acids, acylcarnitines, hexoses, biogenic amines, and lipids in plasma, urine, and tissues was developed, combining LC-MS with FIA-MS analysis. Traditionally, quantitative analyses have been carried out on triple quadrupole MS instruments using MRM technology. The high resolution, accurate mass (HRAM) technology on OrbitrapTM instruments, on the other hand, has predominantly been used in profiling studies. Here, we investigate the coupling of a standardized, quantitative assay with the OrbitrapTM technology to demonstrate the power of multiplexed quantitative metabolome analysis, the impact of improved resolution, and increased metabolite coverage.
1 BIOCRATES Life Sciences AG, Innsbruck, Austria
2 Thermo Fisher Scientific, San Jose, CA, USA