on 05-01-201210:46 AM - edited on 10-15-202111:37 AM by AnalyteGuru
Lössner C, Blackstock W, Gunaratne J. J Am Soc Mass Spectrom. 2012 Jan;23(1):186-9. Pulsed Q collision induced dissociation (PQD) was introduced for isobaric tag quantification on linear ion traps to circumvent the problem of the low-mass cut-off for collision induced dissociation (CID). Unfortunately, fragmentation efficiency is compromised and PQD has found limited use for identification as well as quantification. We demonstrate that PQD has a comparable peptide identification performance to CID on dual-pressure linear ion traps, opening the potential for wider use of isobaric tag quantification on this new generation of linear ion traps.