Enabling Mass Spectrometric Analysis of Intact Proteins in Native Conditions on A Hybrid Quadrupole-Orbitrap Mass Spectrometer

[Orbitrap_SciLib]

Scheffler K, Damoc E, Bailey A, Josephs J.
ASMS 2016 Poster
We have successfully implemented the High Mass Range Mode on the Q Exactive Plus and Q Exactive HF mass spectrometers allowing for mass detection up to m/z 8000. This new operating mode extends the instrument's capabilities to cover all three major workflows for BioPharma characterization. Desolvation/declustering conditions, ions transfer and trapping have been optimized to allow for improved sensitivity in HMR mode for resolution settings as high as 70K. Critical parameters for online LC-MS analyses under native conditions are capillary and probe heater temperatures as key factors in supporting desolvation/declustering.


Thermo Fisher Scientific