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Elucidation of Phosphatidylcholine Composition in Krill Oil Extracted from Euphausia superba

Reputable Mentor II
Reputable Mentor II
Winther B, Hoem N, Berge K, Reubsaet L.
Lipids. 2011 Jan;46(1):25-36.
High performance liquid chromatography-electrospray tandem mass spectrometry was used to elucidate the phospholipids in krill oil extracted from Euphausia superba, an emergin source for human nutritional supplements. This study was carried out in order to map the species of the choline-containing phospholipid classes: phosphatidylcholine and lyso-phosphatidylcholine. In addition, the prevelant phosphatidylcholine class was quantified and the results compared with prior analysis.The qualification was performed with separation on a reverse phase chromatography column, while the quantification was obtained with class separation on a normal phase chromatography column. An Orbitrap system was used for the detection and pulsed-Q disassociation fragmentation was utilized for the identification of the species. An asymmetrical exclusion list was applied for the detection of phospholipid species of lower concentration, significantly improving the number of species observed. A total of 69 choline-containing were detected, whereof 60 phosphatidylcholine substances, among others seven with probable omega-3 fatty-acids in both sn-1 and sn-2. The phosphatidylcholine concentration was estimated to be 34 +/- 5 g/100g oil (n=5). These results confirm the complexity of the phospholipid composition of krill oil, and the presence of long chained, heavily unsaturated fatty acids.
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway.
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