on 05-01-201210:54 AM - edited on 10-15-202110:25 AM by Closed Account
Hornshaw M, Morrice N. Scientific Poster The analysis of phosphopeptides on a linear ion trap mass spectrometer has typically been performed by Data Dependent MS/MS followed by MS3 of the putative neutral loss peak if observed in the MS/MS spectrum (neutral loss MS3). The strength of this approach is the sensitivity in full scan MSn of linear ion trap technology and the characteristically strong neutral loss peak serving to flag the MS/MS spectrum as potentially belonging to a phosphopeptide. However, two novel techniques have recently been developed that can be applied to the analysis of protein phosphorylation. Here we seek to better understand the utility of multi-stage activation (MSA) and electron transfer dissociation (ETD) applied to the analysis of sites of phosphorylation.
Thermo Fisher Scientific, Hemel Hempstead, UK
MRC Protein Phosphorylation Unit, University of Dundee, Dundee, UK