on 06-07-201204:11 AM - edited on 10-15-202111:57 AM by Closed Account
Kiyonami R, Ravnsborg C, Madsen O, Zabrouskov V. Technical Note 63546 The EASY-Spray concept provides an integrated, temperature-controlled column-emitter solution that requires a user to make a single nanoViper connection between the LC and the MS source to achieve exceptional nanoflow LC/MS performance. EASY-Spray columns eliminate dead volumes, assuring narrow peaks and excellent results. EASY-Spray columns are available in 15 cm, 25 cm and 50 cm lengths for high separation efficiency and resolution to maximize peptide coverage.
In contrast, chip-based microfluidic systems use short columns with limited resolution and loading capacity and may lack temperature control.
The new EASY-Spray column design provides excellent retention time reproducibility over wide range of flow rates and column temperatures as tested on a single column and in column-to-column tests. The EASY-Spray emitter also demonstrated high robustness. With its ease-of-use, robustness and reliability, the new EASY-Spray source provides state-of-the-art nanoflow HPLC performance for both MS experts and non-experts.