Nanoflow liquid chromatography-mass spectrometry (LC/MS) is widely used for qualitative and quantitative proteomics studies due to the high sensitivity it provides. Nanoflow operation is traditionally unreliable, however. Small imperfections in connections between the tubing, column, high-voltage electrode, and emitter can easily result in poor or irreproducible results. Improper tubing connections cause leaks and large dead volumes that result in substantial peak broadening and thus poor sensitivity. Poor high voltage connections yield poor data due to spray instability.