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AnalyteGuru_KB3
Scientific Advocate II
Scientific Advocate II
Peptide mapping is one of several routine methods to
characterize biopharmaceutical proteins. For research
environments, this technique, if combined with mass
spectrometry (MS), is utilized for the characterization and
confirmation of the primary sequence of monoclonal
antibodies. In addition, peptide mapping can help to
identify, localize, and quantitate post-translational
modifications (PTMs). Peptide mapping methods are often
developed and evaluated with combined UV and MS
detection, to simplify the transfer to routine environments
where UV detection is used alone. In high-throughput
workflows, peptide mapping experiments are performed
for antibody identity confirmation, PTM characterization,
and stability studies.
The new Vanquish Flex UHPLC system features a
quaternary pump
1
for highest application flexibility and
fully biocompatible flow path. In addition, similar to the
Thermo Scientific Vanquish UHPLC system
2
, the sample is
pressurized prior to the injection into the high pressure
flow path. This results in a highly stable flow delivery and
thus significantly improved retention time precision,
increasing the confidence in peak assignment in peptide
mapping experiments with UV detection.
3
Version history
Last update:
‎09-23-2015 01:20 AM
Updated by: