on 07-31-201309:57 AM - edited on 10-15-202111:38 AM by AnalyteGuru
Johnson J, Eliuk S, Golan A, Johnson T, Zabrouskov V, Krogan N ASMS 2013 Poster Note The identification of virus-host protein-protein interactions has emerged as a powerful tool to characterize the mechanisms by which viruses evade and usurp host cellular pathways. We previously described a highly successful analysis of HIV-human protein-protein interactions through affinity purification and mass spectrometry (AP-MS) analyses of cell lines expressing affinity tagged HIV proteins. Here, we demonstrate the power of applying deep coverage, quantitative analysis of HIV-human protein-protein interactions over a time course of infection using a novel hybrid instrument, Thermo ScientificTM Orbitrap FusionTM mass spectrometer, based on mass resolving quadrupole, Orbitrap, collision cell, linear ion trap (Q-OT-qIT) architecture. Clustering time-dependent quantitative interaction profiles rapidly separated specific and non-specific interactions and identified novel protein-protein interactions that were not identified in previous studies.
UCSF, San Francisco, CA; Thermo Fisher Scientific, San Jose, CA