Development of an All-Recombinant Intact Protein Standard for LC & MS Application Development & System Suitability Testing

[Orbitrap_SciLib]

Rosa Viner1, Helene L. Cardasis1, Viktorija Vitkovske2, Shane Bechler1, Lukas Taujenis2, Vikrant Gohil2, Kay Opperman1, John Rogers1, Egle Capkauske2, , Kestutis Bargaila2, Agne Alminaite2, Juozas Siurkus2
International Hupo 2016
In recent years, interest in intact protein analysis by HPLC, LC-MS, and MS/MS has increased significantly. This can be attributed to both improvements to LC and MS hardware, instrument control software, and data processing software, as well as conceptual shifts in how we can best address and answer biological questions given these emerging commercially available capabilities. Having witnessed the explosive growth of bottomup proteomics and the subsequent evolution of high-quality, widely accessible standards to normalize platform performance in time and space, and assist with method development for new applications, we recognize a similar need for the Top-down proteomics field. Here, we describe the development of a multi-purpose intact protein standard for LC, LC-MS, and LC-MS/MS quality control and application development.


1 Thermo Fisher Scientific, USA 2 Thermo Fisher Scientific, Lithuania