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Development of a Robust and Reproducible Global Plasma Proteome Profiling Workflow for Clinical Research

Reputable Mentor II
Reputable Mentor II
Scott Peterman1, Emily Chen1, David Sarracino1, Amol Prakash2, and Ken Miller1
ASMS 2018
Purpose: Increase the routine plasma proteome profiling capabilities while maintaining 1 hour sample injection cycles and maximizing system robustness. Methods: The developed method modified the chromatographic separation by incorporating analytical flow UHPLC pumps, a PLRP-S trapping column, and 50-cm analytical column to maximize loading and peak capacities. Results: The alternative chromatographic method enabled significantly greater amounts of plasma digest to be evaluated reproducibly while mitigating adverse chromatographic and mass spectral performance. Over 350 proteins were routinely quantified using a 52-minute method.

1 Thermo Fisher Scientific PMSC, Cambridge, MA, USA 2 Optys Technologies, Shrewsbury, MA, USA
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Last update:
‎10-15-2021 11:31 AM
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