Scott Peterman1, Emily Chen1, David Sarracino1, Amol Prakash2, and Ken Miller1
ASMS 2018
Purpose: Increase the routine plasma proteome profiling capabilities while maintaining 1 hour sample injection cycles and maximizing system robustness.
Methods: The developed method modified the chromatographic separation by incorporating analytical flow UHPLC pumps, a PLRP-S trapping column, and 50-cm analytical column to maximize loading and peak capacities.
Results: The alternative chromatographic method enabled significantly greater amounts of plasma digest to be evaluated reproducibly while mitigating adverse chromatographic and mass spectral performance. Over 350 proteins were routinely quantified using a 52-minute method.
1 Thermo Fisher Scientific PMSC, Cambridge, MA, USA
2 Optys Technologies, Shrewsbury, MA, USA
United States
