on 07-08-201606:03 AM - edited on 11-09-202101:16 AM by usermigration2
Scott Peterman, David Sarracino, Amol Prakash, Bryan Krastins, Gregory Byram, Gouri Vadali, Maryann Vogelsang ASMS 2016 Poster Note 64441 Purpose: Develop a comprehensive translational proteomics workflow for urine combining sample preparation, data acquisition, and processing.
Methods: Performed pSMART data acquisition to sample UHPLC peaks for increased protein/peptide content generation per unit time. Incorporate unique data processing strategies in Pinnacle software for automated qualitative and quantitative analysis.
Results: The experimental method using pSMART generated over 55% more peptides consistently sampled per injection. Unique data processing strategies in the Pinnacle software significantly increased the protein sequence coverage compared to Sequest searching for N- and C-terminal truncation analysis, which is key for urine proteomics.
Thermo Fisher Scientific BRIMS, Cambridge, MA; Optys Technology Inc. Philadelphia, PA, and Nuclea Biosciences, Cambridge, MA