Helene L. Cardasis1, Rosa Viner2, Vikrant Gohil1, Kay Opperman3, Shane Bechler4, John Rogers3, Kelly Flook4, Egle Capkauske5, Kestutis Bargaila5, Agne Alminaite5, Viktorija Vitkovske5, Paul Haney3
Purpose : develop a high quality, reproducible intact protein standard for LC-MS QC and application development.
Methods : expression and purification of recombinant proteins that meet MW, m/z distribution, purity, stability, reproducibility requirements of a sample used for LC and MS QC.
Results: a high quality, recombinant intact protein mixture for LC-MS quality control and application development was developed.
1 - Thermo Fisher Scientific, Cambridge, MA 02139
2- Thermo Fisher Scientific, San Jose, CA 95134
3- Thermo Fisher Scientific, Rockford, IL 61101
4- Thermo Fisher Scientific, Sunnyvale, CA 94085
5- Thermo Fisher Scientific, Vilnius, Lithuania LT-02241