Showing results for 
Search instead for 
Did you mean: 

Development and validation of an open screening method for diuretics, stimulants and selected compounds in human urine by UHPLC–HRMS for doping control

Reputable Mentor II
Reputable Mentor II
Girón AJ, Deventer K, Roels K, Van Eenoo P.
Anal Chim Acta. 2012 Apr 6;721:137-46.
A new doping control screening method for the analysis of diuretics and stimulants using ultra high pressure liquid chromatography–high resolution Orbitrap mass spectrometry has been developed. The screening was performed in full scan MS with scan-to-scan polarity switching which allowed to detect more than 120 target analytes. Sample preparation was limited to 10-fold dilution of the urine into the internal standard solution followed by injection. Total run time per sample was 10 min. Validation of the method yielded detection limits for diuretics between 25 and 250 ng mL−1 and for stimulants between 5 and 500 ng mL−1. The screening method has been implemented in routine doping control.
Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain.
Version history
Last update:
‎10-15-2021 08:03 AM
Updated by: