Determination of the natural abundance δ15N of taurine by gas chromatography–isotope ratio measurement mass spectrometry
Illa Tea*, Ingrid Antheaume, Jorick Besnard and Richard J. Robins
Elucidation of Biosynthesis by Isotopic Spectrometry Group, Unit for Interdisciplinary Chemistry: Synthesis-Analysis-Modelling University of Nantes–CNRS UMR6230, 2 rue de la Houssinie`re, BP 92208, F-44322 Nantes, France
Rapid Communications in Mass Spectrometry (2010), V24, pp3380–3386, doi: 10.1002/rcm.4784
The measurement of the nitrogen isotope ratio of taurine (2-aminoethanesulphonic acid) in biological samples has a large number of potential applications. Taurine is a small water-soluble molecule which is notoriously difficult to analyze due to its polarity and functionality. A method is described which allows the determination of the natural abundance d15N values of taurine and structural analogues, such as 3-amino-1-propanesulphonic acid (APSA), by isotope ratio mass spectrometry interfaced to gas chromatography (GC-irm-MS).
- IRMS
- Delta V Advantage
- Flash 1112 HT
- GC
- 15N
- Taurine
United States
