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Team TFS
Team TFS
Elucidation of Biosynthesis by Isotopic Spectrometry Group, Unit for Interdisciplinary Chemistry: Synthesis-Analysis- Modelling (CEISAM), University of Nantes–CNRS UMR6230, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex, France
Analytical and Bioanalytical Chemistry (2011), V401(4), pp1263-71, doi: 10.1007/s00216-011-5182-1
Determination of the concentration of nitrogenous bio-organic compounds using an isotope ratio mass spectrometer operating in continuous flow mode
Illa Tea, Katarzyna Kosieradzka, Ingrid Antheaume, Emmanuel Gentil and Richard J. Robins
The quality of the determination of compound-specific isotopic content at natural abundance by gas chromatography-isotope ratio measurement-mass spectrometry (GC-irm-MS) relies on the stability of the voltage generated by the ion detector Faraday cages. The application of GC-irm-MS to the determination of delta13C (‰) and delta15N (‰) is now routine. However, for numerous applications, it is necessary to determine both the isotope content (delta15N) and the quantity (in micromoles) of analyte present. We now show that it is possible for nitrogen-containing compounds to measure how much analyte is present with an irm mass spectrometer linked to a GC by exploiting the integrated N(2) total ion current intensity (Vs) generated by measuring the (15)N/(14)N isotope ratio.
  • Nitrogen isotopes
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