Determination of Meat Authenticity Using a Comprehensive Targeted Proteomic Strategy and High-Resolution Mass Spectrometry

[Orbitrap_SciLib]

Orduna A, Beaudry F.
Scientific Poster
Purpose: Describe a sensitive, robust HRAM LC-MS method to detect and identify marker proteins in raw meat samples. Methods: Well-defined proteogenomic annotation, carefully selected surrogate tryptic peptides, and analysis using a hybrid quadrupole-Orbitrap MS were integrated. Results: The targeted method allowed for the detection of undesired meat species down to 1% (w/w) of the entire sample. Myoglobin tryptic peptides from each species were detected with an observed m/z below 1.3 ppm.


University de Montreal