on 08-10-201203:23 AM - edited on 10-15-202110:27 AM by AnalyteGuru
Dauly C, Garcia P, Louette J, Cormant F, Oppermann M, Popot MA, Bonnaire Y, Bailly-Chouriberry L. ASMS 2012 Video Poster Recombinant human erythropoietin (rHuEPO) is a 30-34 kDa glycoprotein banned by
racing authorities. To differentiate between horse endogenous EPO and rHuEPOs,
methods employing LC-MS/MS or isoelectric focusing (IEF) with double-blotting have
been developed. However, the short confirmation-time window of the compound in
plasma remains a problem for doping-control laboratories in horse racing. In this study,
a new sample preparation technique using disposable anti-EPO monolith columns was
combined with nano-LC-MS/MS analysis on a high-resolution Orbitrap mass analyzer.
The approach enabled the confirmation and quantification of the rHuEPO target
peptides in a single analytical run, with high specificity, sensitivity and resolution.