on 06-20-202003:41 PM - edited on 10-15-202111:19 AM by AnalyteGuru
Amanda Souza, Ioanna Ntai, Tatjana Talamantes and Ralf Tautenhahn
Purpose: Develop an integrated mass spectrometry-based workflow for stable isotope labeling (SIL) in untargeted metabolomics covering data acquisition and analysis in a single experiment.
Methods: Metabolites from E. coli extracts consisting of unlabeled, 13C-labeled and mixtures
rendering incorporation levels of 10%, 25%, and 50% were analyzed with reversed phase separation and a Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer. SIL data were processed using an untargeted approach including detection of labeled metabolites and unknown annotation.
Results: Untargeted metabolomics was applied to an SIL experiment. Confident formulas were generated using higher resolution settings along with accurate mass measurements enabling the detection of 13C-incorporated isotopologues in labeled samples. Annotation capability facilitated detection of unexpected metabolites with label incorporation.
Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, CA, USA, 95134