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Reputable Mentor II
Reputable Mentor II
Amanda Souza, Ioanna Ntai, Tatjana Talamantes and Ralf Tautenhahn

Purpose: Develop an integrated mass spectrometry-based workflow for stable isotope labeling (SIL) in untargeted metabolomics covering data acquisition and analysis in a single experiment. Methods: Metabolites from E. coli extracts consisting of unlabeled, 13C-labeled and mixtures rendering incorporation levels of 10%, 25%, and 50% were analyzed with reversed phase separation and a Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer. SIL data were processed using an untargeted approach including detection of labeled metabolites and unknown annotation. Results: Untargeted metabolomics was applied to an SIL experiment. Confident formulas were generated using higher resolution settings along with accurate mass measurements enabling the detection of 13C-incorporated isotopologues in labeled samples. Annotation capability facilitated detection of unexpected metabolites with label incorporation.

Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, CA, USA, 95134
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Last update:
‎10-15-2021 11:19 AM
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