Egertson J.
Tutorial
This brief tutorial describes how to generate a DIA method using the Skyline
Targeted Proteomics Enivronment suitable for running on the Q-Exactive. Skyline generates an optimized list of isolation windows to cover a m/z range of interest which is then entered into the Q-Exactive method editor. Here, we create a method covering 500-900 m/z with 20 m/z wide isolation windows. At the end of the tutorial, there is a note on how to generate a multiplexed method as in
http://www.ncbi.nlm.nih.gov/pubmed/23793237. This tutorial was written for use with Skyline 2.1 and Q-Exactive software version 2.2 SP1. Please post any questions, comments, or suggestions on the Skyline support board at
https://skyline.gs.washington.edu/labkey/project/home/support/begin.view.
University of Washington
United States
