Eliuk S, Viner R. Webinar and Downloadable Presentation with Speaker Notes Phosphorylation and glycosylation are two common posttranslational modifications (PTMs) of proteins that perform critical roles in the regulation of many cellular processes including cell cycle, signal transduction pathways, recognition, gene expression and cellular immunity.
Mass spectrometry is a powerful tool for the detection and identification of PTMs. Comprehensive characterization of proteins include localization of the modification site, characterization of structures and quantification of the various modified forms to fully understand the effect on protein function. Electron Transfer Dissociation, (ETD) is a fragmentation technique that has been used enthusiastically for peptide identification, localization of labile post-translational modifications (PTMs) and sequence polymorphisms.
This webinar demonstrates the advantages of using a novel Orbitrap mass spectrometer for qualitative and quantitative PTM analysis. The novel arrangement of the three mass analyzers plus advances in software not only increases the rate of high quality ETD spectra, but also the ease of routinely obtaining high quality ETD data.
Enhanced peptide ID will be discussed as well as PTM identification and localization for both phosphorylation and glycosylation.