Complete characterization of a lysine-linked antibody drug conjugate by native LC/MS intact mass analysis and peptide mapping

[Orbitrap_SciLib]

Aaron O. Bailey,1 Stephane Houel,1 Kai Scheffler,2 Eugen Damoc,3 Jennifer Sutton,1 Jonathan L. Josephs
APPLICATION NOTE 72511
To perform complete characterization of the lysine-linked antibody drug conjugate (ADC) trastuzumab emtansine using both intact mass and peptide mapping approaches with a single benchtop mass spectrometer. Critical quality attribute (CQA) measurements include drug-to-antibody ratio (DAR) and glycosylation pattern via intact mass analysis, and conjugation site localization via peptide mapping.


1 Thermo Fisher Scientific 1 San Jose, CA, USA 2 Dreieich, Germany 3 Bremen, Germany