on 06-03-201601:00 PM - edited on 10-15-202111:44 AM by Closed Account
Bailey A, Damoc E, Houel S, Scheffler K, Josephs J. ASMS 2016 Poster We have modified the instrument control software of a benchtop quadrupole-Orbitrap mass spectrometer to add native MS capability. In this study we demonstrate complete characterization of Brentuximab vedotin, a cysteine-linked ADC, which requires native MS conditions for intact analysis. We demonstrate preservation of non-covalent bonding of antibody subunits during electrospray ionization. HMR mode can be turned off for peptide mapping. We use trypsin peptide mapping approach with HCD fragmentation to achieve 99% coverage of the Brentuximab vedotin sequence using a single LC-MS analysis of a 90 min reverse phase gradient. Finally, we demonstrate that signature ions specific for HCD fragmentation of Brentuximab vedotin can be utilized to increase MS/MS assignment confidence.