Comparison and Optimization of First and Second Generation Quadrupole Dual Cell Linear Ion Trap Orbitrap MS for Glycopeptide Analysis

[Orbitrap_SciLib]

Julian Saba1,2, Sergei Snovida3, Christa Feasley4

Purpose: To optimize instrument parameters for the Thermo Scientific™ Orbitrap Fusion™ Lumos™ MS and compare performance against the Thermo Scientific™ Orbitrap Fusion™ MS for intact glycopeptide analysis. Methods: Glycopeptides enriched from various sources were analyzed on the Orbitrap Fusion Lumos MS and Orbitrap Fusion mass spectrometers. Multiple instrumental parameters were tested to maximize intact glycopeptide identifications. Data analysis were performed using Byonic™ software. Results: Improvement in performance for intact glycopeptide analysis was observed on the Orbitrap Fusion Lumos MS relative to the Orbitrap Fusion MS.


1Thermo Fisher Scientific, San Jose, CA, USA 2Thermo Fisher Scientific, Mississauga, ON, Canada 3Thermo Fisher Scientific, Rockford, IL, USA 4Thermo Fisher Scientific, West Palm Beach, FL, USA;