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Orbitrap_SciLib
Reputable Mentor II
Reputable Mentor II
Ejsing CS, Moehring T, Bahr U, Duchoslav E, Karas M, Simons K, Shevchenko A.
J Mass Spectrom. 2006 Mar;41(3):372-89.
The yeast Saccharomyces cerevisiae synthesizes three classes of sphingolipids: inositolphosphoceramides (IPCs), mannosyl-inositolphosphoceramides (MIPCs), and mannosyl-diinositolphosphoceramides (M(IP)2C). Tandem mass spectrometry of their molecular anions on a hybrid quadrupole time-of-flight (QqTOF) instrument produced fragments of inositol-containing head groups, which were specific for each lipid class. MSn analysis performed on a hybrid linear ion trap–orbitrap (LTQ Orbitrap) mass spectrometer with better than 3 ppm mass accuracy identified fragment ions specific for the amide-linked fatty acid and the long chain base moieties in individual molecular species. By selecting m/z of class-specific fragment ions for multiple precursor ion scanning, we profiled yeast sphingolipids in total lipid extracts on a QqTOF mass spectrometer. Thus, a combination of QqTOF and LTQ Orbitrap mass spectrometry lends itself to rapid, comprehensive and structure-specific profiling of the molecular composition of sphingolipids and glycerophospholipids in important model organisms, such as fungi and plants.

http://onlinelibrary.wiley.com/doi/10.1002/jms.997/abstract
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
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