Vitamins are essential organic compounds that an organism needs in limited amounts for normal growth and health. As the organism is unable to synthesize these compounds, they must be obtained through the diet. Vitamin deficiency can lead to disease. Although supplementation is important for the treatment of certain health problems, whether it is of nutritional benefit when used by otherwise healthy people remains unresolved. Low molecular weight antioxidants are organic compounds that prevent key biomolecules from undergoing oxidative damage. They include vitamins as well as non-essential molecules obtained from the diet or synthesized by the organism.This notebook describes chromatography methods for analyzing various vitamins and vitamin mixtures.