A sugar substitute is a food additive that duplicates the sweetness of sugar in taste, but with fewer calories. Some sugar substitutes are natural while others are synthetic. Those that are not natural are, in general, called artificial sweeteners.Six intensely sweet sugar substitutes are approved for use in the United States: acesulfame potassium, aspartame, neotame, saccharin, stevia, and sucralose. This notebook describes chromatography methods for analyzing these sugar substitues.