The work presented herein summarizes the results of a chromatographic characterization study of HILIC stationary phases involving ten silica- based columns, including unmodified silica, amino, diol, anion exchanger, and zwitterionic materials, and a porous graphitic carbon (PGC) column. The column characterization methodology allowed the identification
and understanding of primary and secondary retention mechanisms and the classification of the HILIC stationary phases according to their chromatographic properties. This ultimately can be used as a column selection tool during method development in HILIC separations.
United States
