Vogelsang MS, Prakash A, Sarracino D, Vadali G, Peterman S, Frewen B, Nussupbekova A, Smagul A, Zhao Y, Ahmad S, Byram G, Krastins B, Lunyak VV, Lopez MF.
PN-64144
Purpose: We have completed a label-free quantitative global profiling and targeted
analysis of the cardiac proteome in aging mice using a novel data acquisition method,
pSMART.
Methods: The pSMART method was used to acquire qualitative and quantitative data
using one HR/AM MS and a series of narrow DIA mass windows. Initial
characterization experiments using unbiased DDA facilitated the building of a detailed
spectral library which was used for qualitative scoring for the DIA data.
Results: Our pSMART strategy resulted in 30% more peptide identifications per run
than a standard DDA run. Additionally, using pSMART, we are able to confirm MS1
quantitation at low abundance levels with MS/MS for each peptide. This novel
acquisition enabled quantitation of previously identified peptides as well as novel
putative targets of aging. By identifying and quantifying more targets, we were able to
better characterize the dynamic proteomic changes of cardio-dysfunction in aging
mice.
BRIMS, Buck Institute for Age Research, Kazakh Medical University
United States
