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Characterizing Qualitative and Quantitative Global Changes in the Aging Heart Using pSMART, a Novel Acquisition Method

Reputable Mentor II
Reputable Mentor II
Vogelsang MS, Prakash A, Sarracino D, Vadali G, Peterman S, Frewen B, Nussupbekova A, Smagul A, Zhao Y, Ahmad S, Byram G, Krastins B, Lunyak VV, Lopez MF.
Purpose: We have completed a label-free quantitative global profiling and targeted analysis of the cardiac proteome in aging mice using a novel data acquisition method, pSMART. Methods: The pSMART method was used to acquire qualitative and quantitative data using one HR/AM MS and a series of narrow DIA mass windows. Initial characterization experiments using unbiased DDA facilitated the building of a detailed spectral library which was used for qualitative scoring for the DIA data. Results: Our pSMART strategy resulted in 30% more peptide identifications per run than a standard DDA run. Additionally, using pSMART, we are able to confirm MS1 quantitation at low abundance levels with MS/MS for each peptide. This novel acquisition enabled quantitation of previously identified peptides as well as novel putative targets of aging. By identifying and quantifying more targets, we were able to better characterize the dynamic proteomic changes of cardio-dysfunction in aging mice.

BRIMS, Buck Institute for Age Research, Kazakh Medical University
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Last update:
‎10-15-2021 11:36 AM
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